Brand: MOLECULAR DEVICES. used. We would like to show you a description here but the site won’t allow us. Robust, high-value microplate readers that can run samples. 2 nm 光度量范围 0-4. 15 mmol). The VersaMax and SpectraMax 190 read 96-well microplates. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. For very concentrated samples, use a minimum…200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 00 0 bids 2d 20h. No accessories are needed and SoftMax® Pro Software. Id3 spectramax reader molecular microplate multi mode devices vwr std. 00. The SpectraMax 190 reader is the tool for DNA analysis. View our entire inventory of New Or Used Roadtrek RVs. Auction Details Bio + Laboratory Equipment Expo. 17247 MOLECULAR DEVICES MICROPLATE SPECTROPHOTOMETER SPECTRAMAX 190. Cell viability was denoted with relative growth rate (RGR%), which was calculated by using. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. The . SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. . For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. 0200-7027. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 7°C (gradient heating design). Measurement of Nitric Oxide. Example: [Drawer]. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area. The equation that allows one to calculate absorbance from % transmittance is. The SpectraMax® 190 UV-Vis Microplate Spectrophotometer from Molecular Devices is ideal for life science applications, especially DNA analysis. A. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The SPECTRAmax 340PC can mea-The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed . Example: [Drawer]. Each sample has a discrete sample beam and reference. SpectraMax Injector Cartridge with SmartInject™ Technology. These robust readers have been placed in labs from Antarctica to the. ®User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] mg/ml, 80 μl) was incubated at 25°C in the dark for 30 min, then the absorbance recorded at 517 nm (SpectraMax 190 Microplate Reader, USA). 7 and HaCaT cells were pretreated with MAEO at 12. Description 1. SPECTRAmax® 190. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. We enable science by offering product choice, services, process excellence and our people make it happen. . 006 O. . Performance Specifications . The absorbance was measured using ELISA analytical instruments SpectraMax 190 (Thermo Fisher Scientific, CA, United States) at a wavelength of 450 nm. . Wavelength Range: 190 - 850 nm. Microplate Reader, For Lab. Here, we demonstrate several different ways the ABS Plus reader, in combination with the SpectraDrop™ Micro-Volume Microplate and SoftMax® Pro Software, can quantitate double-stranded DNA and bovine serum albumin (BSA. The SpectraMax Plus 384 reader is equipped with an innovation patented by Molecular Devices, the PathCheck® sensor, which measures the depth of a sample in a microplate by optical path length. SpectraMax 190 Absorbance Microplate Reader with laptop and software Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Ultraviolet transparent multiwell plates from Greiner Highly recommended for measurements of DNA and protein concentrations at 260 nm or 280 nm, respectively By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. For more information, please visit us at 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 0200-6263. 1 in stock. SpectraMax 190 Absorbance Microplate Reader with laptop and software Enables data acquisition in endpoint, kinetic, and spectral scan. Temperature control and shaking come as standard. Spectramax Plus 384 Spectramax 190 Spectramax 340PC384 Versamax Microplate Spectrophotometers; A Fluorescence Correlation Spectrometer for Measurements in. S. It measures the optical density (OD) of samples in 96-wellPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Read Cuvette - For the SpectraMax Plus 384, press to initiate the sample read of the cuvette. After 1 h of incubation, the absorbance of each well was measured at A450 nm using a SpectraMax microplate reader (SpectraMax 190, Molecular Devices, USA). Optimized for fluorescence microplate readers. (a) Chemical structures of 1 (HADP) and its analogues 2–4. SpectraMax 190 Absorbance Microplate Reader. The decrease in absorbance due to decomposition of H 2 O 2 was monitored at 240 nm in a SpectraMax 190 microtitre plate-reader. To contact technical support by phone in the U. 0 out of 5 $ 4,499-20%. Part # 0112-0085 Rev. Capable of detecting DNA down to 25 ng. SpectraMax and VersaMax . Absorption was measured at 450 nm 24 h after premix added using SpectraMax 190 (Molecular Devices, LLC, Sunnyvale, CA, USA) to produce a cell growth curve. 30% phenol. SpectraMax® 340PC384. On LabX buyers can find. Molecular Devices Spectramax 190 Absorbance Microplate Reader. The technology allows automatic adjustment of good absorbance to a tube equivalent path length of 1 cm, converted to 96 or 384 tubes. A four‐parameter logistic function was used to fit the curve and determine unknown concentrations with the software. After this date, all service and support will be on a reasonable effort basis At this time, we are happy to help you upgrade to the SpectraMax® ABS or ABS Plus Microplate Readers which containThe solubility of compounds was measured using a SPECTRAMAX 190 (Molecular Devices, San Jose, CA, USA). SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. please click here. For very concentrated samples, use aBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. 5 mM −1 ∙cm −1) in a SpectraMax 190 plate reader (Molecular Devices). spectramax quickdrop micro-volume spectrophotometer, quantitate micro-volume, quickdrop spectrophotometer specifications, nanodrop, low-volume quantitation, dna, protein, rna, oligo, spectrophotometer, uv/vis. item 3 Molecular Devices SpectraMax 190 Microplate Spectrophotometer Molecular Devices SpectraMax 190 Microplate Spectrophotometer . Price: $2,200. Note: Due to. SpectraMax® 190. Bookings are free, but you will need to use iLab to book. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Spectramax 190 User Manual, Metrix St5484e Manual, Cm2020b Manual, Dek 5650 Manual, Manual De Sony Vegas 6, Manual For Sonicwall Tz 100, Saflok 6000 Manual one-document-company 4. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]®Plus384addstheabilitytoread cuvettes. It measures the optical density (OD) of samples in 96-well SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Prepare your iD series instrument for shipment. SpectraMax 190 and Spect FRET fluorescence, top- le-Mode Detection Fluorescence Sir and bottom-read, cell-based assay, lal injection in both 96- and 384-well lates, excellent sensitivity, Autorinse range, du microp 0+ pre-defined protocols; lac compatible based (visible 405-750 nm)The obtained mixture was reacted in a boiling water bath for 20 min, and then, cooled down immediately. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for. Lower limits of detection for ATP determined using the ATPlite . 8 ng/mL. Molecular Devices technical support. A wide linear dynamic range ensures accurate assay results across a broad span of sample types. We have 1 Molecular Devices SpectraMax 190 manual available for free PDF download: User Manual . The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 1step assay with SpectraMax microplate readers. 305 reference level saturation. 190-SC - SpectraMax 190 Microplate Reader with 1-Year Service Contract - SpectraMax 190 Absorbance Plate Reader, Molecular Devices - Each מותג: Molecular Devices 36,002. The blood was centrifuged at 3000 rpm for 15. Add to Cart Buy. Note: If you install the SpectraMax MiniMax 300 Imaging Cytometer, do not use this procedure. Product Specs; Item SpectraMax 190 Absorbance Microplate Reader; Company Molecular Devices LLC; This product is no longer available on Biocompare. Find many great new & used options and get the best deals for Molecular Devices SpectraMAX 190 Microplate Reader at the best online prices at eBay! Free shipping for many products!Mini-Bead Beater (BioSpec Products); fluorimeter microplate reader (SpectraMax. SKU: 17247 Category: Laboratory Equip. Figure 1. The dichloromethane, methanol, and hexane extracts were eluted four times and the absorbance spectra of the samples was measured at a range of wavelengths from 200 to 750 nm. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. . Filter wheels can be shipped preloaded. The analysis was performed in triplicate and the results were expressed as mg gallic acid equivalent per g of extract (mg GAE/g). The corrected absorbance is within 5% of the. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. absorbance readers. 3. The mixture was shaken and incubated for 30 min at room temperature in the dark. Read - For the SpectraMax 190 and SpectraMax 340PC384, press to initiate the plate read when you operate the instrument in stand alone mode. Minimize sample volume while retaining sensitivity with the SpectraDrop Micro-Volume Microplate. Spectramax® id3 multi-mode microplate reader, molecular devicesMolecular devices showcases spectramax I3x spectramax pdfSpectramax® i3:. According to the manufacturer, the sensitivity of this assay is 5 ng/ml and has less than 1% cross. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. A SOFTMAX® PRO SOFTWARE Version 5 for Mac® and Windows® User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. Examples: “Press carefully to engage,” “Do not. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The multi-channel design truly mimics a dual-beam spectrophotometer. Highly selective for dsDNA over ssDNA or RNA. 2 - 3. 3™ software instructions View of back of Spectra Max 190 . DNA provides the same sensitivity as a spectrophotometer. Service parts for these models are not being produced and are no longer in stock. The temperature in the plate chamber is ambient and isothermal. RvTrader. 1. The multi-channel design truly mimics a. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Part # 0112-0085 Rev. Service Contracts, Preventative Maintenance and Performance. The ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. These models can no longer be repaired. Compounds were tested for inhibition of 3CL pro following a similar protocol with several small modifications. Luminescence. no. It measures the optical density (OD) of samples in 96-wellLow-volume, high-throughput DNA and protein detection on the SpectraMax ABS Plus Microplate Reader. Ultraviolet transparent multiwell plates from Greiner; Highly recommended for measurements of DNA and protein concentrations at. Description 1. After incubating for 10 min at room temperature, the absorbance was measured at 510 nm by SpectraMax 190 Microplate Reader (Molecular Devices, San Jose, CA). The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. ®iD5. 1) Select the correct plate and the wells that you want to measure. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. The protein contents of the samples were determined with the BCA Protein Assay Kit. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. . Wavelength Range: 190 - 850 nm. The negative control was cells in the fresh culture medium, and the positive control was cells in the culture medium that contained 0. Determine concentration using the Beer-Lambert LawThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. In vitro blood test. Wavelength Bandwidth. Analysis of pro-inflammatory macrophage phenotypic response to NAM and RSV. #3635 or Greiner cat. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. 000 (OD). We assessed the expression of pro- and anti-inflammatory genes expressed by macrophages, including. Check Details. The VersaMax and SpectraMax 190 read 96-well microplates. Absorbance was calculated at 420 nm in a 96-well ELISA plate using an “absorbance microplate reader” (SpectraMax 190, designed in USA, manufactured in China). Briefly, the. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe VersaMax and SpectraMax 190 read 96-well microplates. 0 out of 5 $ 4,499-5%. For very concentrated samples, use a minimum0200-6262. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Description 1. SOFTmax PRO. NO assayIMI detection was conducted using the Eurofins Imidacloprid ELISA kit (Eurofins Abraxis, Warminster, PA, USA) following the manufacturer’s instructions for extraction and quantification of IMI residues in leaf tissue and using a spectrophotometer (SpectraMax 190, Molecular Devices, San Jose, CA, USA). Protein concentrations were calculated using polynomial 2nd analysis from the protein standards. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. The soluble PAs were quantified by the DMACA method. Multi-mode Microplate Readers. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. no. Gemini™. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. Examples: “Press carefully to engage,” “ Do not press down. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. 118 Gemini XPS Fluorescence Microplate Reader . All service plan coverage will end by December 31, 2028. The VersaMax and SpectraMax 190 read 96-well microplates. Italic and boldface type are used for emphasis. 1) This. The optical density at 450 nm was measured with the SpectraMax 190 Microplate Reader (Molecular Devices). All the measurements were taken in triplicate and the mean values were calculated. The VersaMax and SpectraMax 190 read 96-well microplates. T&Cs & Important Info. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. The MIC was determined by the concentration where there was an obvious clear-cut decrease in the absorbance. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. Currently unavailable. Each sample has a discrete sample beam and reference beam so that each well is measured directly. For aqueous solutions, our patented* PathCheck® sensor. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. DNA provides the same sensitivity as a spectrophotometer. ScanLater Western Blot Detection Cartridge. Lipid accumulation in beige adipocytes treated with rosiglitazone or diosmin was detected by Oil Red O staining and measured at 520 nm by SpectraMax 190 microplate reader (Molecular Devices). The same was done using samples of colloidal chitin to confirm concentrations. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. The SPECTRAmax 190 can accommodate standard 96-well microplates, strip wells, and Þlter-bottom microplates. When connecting for the first time to either the SpectraMax L reader or StakMax system. Description. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. ®System (PerkinElmer) On The SpectraMax ID5 Reader. 6 stars - 1134 reviewsSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. SoftMax Pro Software has been integrated by many leading robotics andSPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $ 3,250. The ELISA signal was measured using the ELISA plate reader SpectraMax 190 (Molecular Devices, Sunnyvale, CA, USA). for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Plus 384 Protocol (MS Word) For more. The fresh whole blood was obtained from SD rats and anticoagulated with 3. 2. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Examples: fiPress carefully to engage,fl fiDo not press down. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Photometric Performance SpectraMax 190 Item Description. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesSpectraMax 190 Absorbance Microplate Reader by Molecular Devices®. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The Spectramax 190 has. ; The top 3 importers of Spectramax are India with 365. The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). Wavelength precision. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. Reads various kinds of 96-wells plates, and is equipped with shaking function. Italic and boldface type are used for emphasis. The SPECTRAmax 190 can accommodate standard 96-well microplates, strip wells, and Þlter-bottom microplates. it should turn freely, without any friction. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. Seller InformationAnytimeLabTrader. SpectraMax ID5 Plate Reader (Molecular Devices) Absorbance. Molecular Devices’ PathCheck® sensor technology. The ABS reader ranges from 340–850 nm in wavelength and the ABS Plus reader ranges from 190–1000 nm, which is equivalent to having 510 or 810 free filters, respectively. The multi-channel design truly mimics a dual-beam spectrophotometer. 4 Figures from 1912 Publications. • If SpectraMax can not communicate with a specific device, it will display a window showing which device and allow for the user to modify the communications settings or emulate the device (ifThe SpectraMax 190 reader is the tool for DNA analysis. For wavelengths in the UV range above 220 nm,. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. Review date: 12 Jun 2017 | SpectraMax Plus 384 Microplate Reader Get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 00 . It measures the optical density (OD) of samples in 96-well Low-volume, high-throughput DNA and protein detection on the SpectraMax ABS Plus Microplate Reader. Page 1Method 66DNA Analysis on the Molecular Devices Spectramax Plus and Spectramax 190 microplate readers Quick Reference Guide Section A: Plate Materials A UV transparent microplate is required for. Temperature control. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The dual-scanning capability can also be used to determine excitation and emission settings for new fluorescent probes. Molecular Devices microplate spectrophotometer, mod SpectraMAX 190, s/n 02552, includes computer. Standard concentrations ranged from 1x10-11 M to 1x10-6 M. Phone Number Login / Register. These filter wheels are used for a host of applications, including color CCD photography, fluorescence microscopy, and photometry. Double-click the unknown device with the yellow warning icon. Add to Cart. Description. ¨The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The 96-well plate was detected by a SpectraMax® 190 microplate reader (Molecular Devices, San Jose, USA) at 490 nm to obtain the relative cell viability. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. TECHNICAL NOTE. 3 mm. Flavonoid Compositions Analysis . TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive measurementsofavarietyofanalytesacrossawiderangeofconcentrations. For very concentrated samples, use a minimumSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. Type: Clear: Add to Cart. DNA provides the same sensitivity as a spectrophotometer. Find many great new & used options and get the best deals for Molecular Devices SpectraMax 190 Absorbance Microplate Reader at the best online prices at eBay! Free shipping for many products!An ELISA reader (SpectraMax 190) was used to measure the absorbance of formazan solution at 490 nm. Optical density was measured at 570 nm with an automatic microplate spectrophotometer (SpectraMax 190, Sunnyvale, USA). Wavelength selection Monochromator tunable in 1 nm increments. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. Introduction. We would like to show you a description here but the site won’t allow us. Title: SpectraDrop Micro Volume Microplate | Molecular Devices Author: Molecular Devices, LLC Subject:We will match our competitor's price! The SpectraMax® 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. 2. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. 2) If you wish to regulate the temperature inside the microplate reading cham-. fl 312 gain calibration failed. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Molecular Devices SpectraDrop™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax® Microplate Readers, with sample volumes as low as 2 µL. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Modereader (SpectraMax 190, Molecular Devices, USA). 3 Figures from 612 Publications Promega. Compatible automation solutions for SpectraMax Readers. 0 nm * Wavelength Repeatability ± 0. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The multi-channel design truly mimics a dual-beam spectrophotometer. I do that by turning the unit off, and turning the wheel. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. . , China). Both microplate and cuvette ports can be qualified. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Examples: “Press carefully to engage,” “ Do not. The standard curve was made using iron (II) sulphate solution (40–0. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- The VersaMax and SpectraMax 190 read 96-well microplates. Molecular Devices SpectraMax 190 Microplate Reader. Read - For the SpectraMax 190 and SpectraMax 340PC384, press to initiate the plate read when you operate the instrument in stand alone mode. 0 nm increments Wavelength bandwidth 2 nm Wavelength accuracy ±1. 00. Molecular Devices SpectraMax 340PC384 Microplate Reader $ 4,000. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. Features and Benefits Corning ® UV-Transparent Microplates are ideal for determining protein and/or nucleic acid concentrations. SOFTmax PRO makes it possible to. Open the catalog to page 1. Model: SpectraMax 250 Condition: Used. The corrected absorbance is within 5% of the value. The SpectraMax 190 Microplate Reader is a high-performance spectrophotometer that can measure DNA absorbance across the microplate with a unique quartz fiber optic system and a PathCheck sensor. Introduction. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. EZ-Cytox (10 µL) was added to each of the wells of the plate and incubated for 1 h. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. , Tokyo, Japan) was added to each well every 24 h. 119. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Add to cart. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. Page 10 SpectraMax i3x Multi-Mode Detection Platform Installation Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. No accessories are needed and SoftMax® Pro. SpectraMax® 340PC384 Microplate Reader for acquiring data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Quercetin was used as the standard (1 mg/ml) solution in order to obtain the calibration curve using a series of concentrations, i. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wella SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). Wavelength Accuracy: < ± 2. The absorbance was read every 30 s for a period of 3 min using a SpectraMax 190 microplate reader. 22 × 10 3 M −1 cm −1). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. After incubation at 37 °C for 15 min, the resulting color reaction was read at 450 nm by a microplate reader (Spectramax 190, Molecular Devices Corporation, Sunnyvale, CA, USA) within 10 min, and the final IL-1 concentration was calculated using a 4-parameter logistic curve fit. RAW 264. A mixture of DPPH (0. 0 out of 5 $ 9,995 $ 9,495. 0 out of 5 $. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader. ®Molecular Devices SpectraMax 190 Microplate Reader. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Italic and boldface type are used for emphasis. The patented multichannel optical design of the SpectraMax® 190. 078 μg/mL), and the results were expressed as μg Fe (II)/g of extract. 0 out of 5 $ 2,499 $ 1,999. The enzyme activity was calculated as nmol NADPH oxidized/min/mg protein (ε = 6. The SpectraTest validationIn this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Three low volume 24-well bottom slides. The SpectraMax 190 Microplate Reader is supplied with SoftMax Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Absorbance was read on a SpectraMax 190 plate reader (Molecular Devices, Wokingham, Berkshire, UK). The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. The optical density (OD) of the samples at 450 nm was measured by a SpectraMax 190, then the LDH activity was further calculated by a relevant formula. SpectraMax® Plus 384. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). ®{"general":{"total":5,"offset":0,"limit":5,"data":[{"key":"label","path":"path","name":"Name","identifier":"Identifier","wavelength-ranges":"Wavelength ranges. The cell pellets were lysed with 200 mmol/L NaOH, and the iron content was determined using the ferrozine assay. Ends from. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. For more sample throughput, the system can The SpectraMax® ABS Plus is a compact UV-Vis absorbance microplate reader ideal for these types of quantitative assays. Streamline GLP/GMP compliance. Protein concentrations were calculated using. 1. Database contains 2 Molecular Devices SpectraMax 190 Manuals (available for free online viewing or downloading in PDF): Operator's manual, Operation &. Molecular Devices LLC 96 well microplate spectrophotometer spectramax 190 96 Well Microplate Spectrophotometer Spectramax 190, supplied by Molecular Devices LLC, used in various techniques. Plate chamber design of the SpectraMax category 1 microplate readers.